Protocol :
1. Load 5 ul ladder for a gel lane, and adjust loading volumeaccording to the width of the agarose gel.
2. Use 1.0 - 2.0 % agarose, and 4 - 10 v/cm, in 1xTAE electrophoresis buffer.
3. Visualize DNA by EB (ethidium bromide) staining, or SYBR Green I.
5x Loading Dye
10 mM Tris - HCl,
5 mM EDTA, pH7.6,
0.03 % Bromophenol blue,
0.03 % Xylene cyanol,
30 % Glycerol.