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Plasmid DNA Purification System

GeneAll Plasmid DNA Purification Systems utilize glass microfiber membranes based on the modified alkaline lysis method.

GeneAll Hybrid-QTM Plasmid Rapidprep with new patented EzClearTM filter provides the alternative methods for standard or rapid preparation of Plasmid DNA depending on plasmid copy number, host strain, culture medium and culture volume.

GeneAll ExfectionTM Plasmid LE (Low Endotoxin) and EF (Endotoxin-Free) kits provide simple and fast method for the purification of plasmid DNA with low endotoxin contaminants. Endotoxins present in the cell membrane of gram-negative bacteria are common contaminants in plasmid preparations and can significantly reduce transfection efficiencies. ExfectionTM series can be used for the transfection of most cell lines through the removal of endotoxins: advanced phase separation and endotoxin removal washing.

GeneAll ExpinTM series provide reliable and fast methods for the purification of fragment DNA from agarose gel and PCR or enzymatic reaction mixtures.
ExpinTM Gel SV takes advantage of glass fiber membrane to recover DNA of 80 bp to 10 kb from most grades of agarose gel in yields reaching 90 %.
ExpinTM PCR SV is used to recover DNA of 100 bp to 10 kb from PCR or enzymatic reaction mixtures and very effective to the removal of PCR primer dimer.
ExpinTM Combo GP kit is the combined product of ExpinTM Gel SV and ExpinTM PCR SV.
ExpinTM CleanUp SV is designed for fast and simple method for purification of fragment DNA of 40 bp to 10 kb from various enzymatic reactions in just 5 minutes.
 

Exfection Plasmid LE

Description

ExfectionTM plasmid LE (Low Endotoxin) and EF(Endotoxin-Free) provide simple and fast method for the purification of plasmid DNA with low endotoxin contaminants.Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli.
It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. These kits use two methods for the removal of endotoxins: advanced phase separation and endotoxin removal washing.
Endotoxin levels can be reduced to 0.1 EU/㎍ with ExfectionTM EF and to 10 EU/㎍ with ExfectionTM LE. Prepared plasmid DNA can be used for the transfection of most of cell lines in addition to most of molecular biological applications.

ab 80,00 EUR
zzgl. 19% MwSt. zzgl. Versand
Hybrid-QTM Plasmid Rapidprep

Description

Hybrid-QTM Plasmid Rapidprep kit provides two methods for easy and rapid preparation of plasmid DNA from the mini scale bacterial cells. Plasmid DNA can be prepared from up to 10 ml of overnight culture by conventional miniprep method with standard protocol. Alternatively, up to 3 ml of sample can be processed by rapid protocol in just 10 minutes with new patented EzClearTM filter and simultaneous processing of multiple samples can be easily performed. Up to 30 ㎍ of pure plasmid can be purified using Hybrid-QTM Plasmid Rapidprep kit and this pure plasmid DNA is ready for PCR, cloning, fluorescent sequencing, synthesis of labeled hybridization probes, cell transfection, electroporation and enzymatic restriction analysis without further manipulation.
ab 51,00 EUR
zzgl. 19% MwSt. zzgl. Versand
Exprep Plasmid SV

Description

ExprepTM Plasmid SV DNA Purification kit provides a rapid and convenient method for the small and medium scale preparations of plasmid DNA from bacterial cells and it is used to isolate and purify any plasmids from any E. coli strains. ExprepTM Plasmid SV eliminates the need of organic solvent extraction and alcohol precipitation, allowing rapid and convenient preparation from many samples simultaneously.
ExprepTM Plasmid SV kit can yield up to 30 ㎍ (mini) of highly purified plasmid DNA and it can be applicable directly for PCR, cloning, automated sequencing, synthesis of labeled hybridization probes and other enzymatic reactions without further manipulation. ExprepTM Plasmid SV Midi kit procedure requires the centrifuge which has a swinging-out bucket and ability of 4,000 ~ 5,000 xg.

ab 59,00 EUR
zzgl. 19% MwSt. zzgl. Versand
Exfection Plasmid EF
ExfectionTM plasmid LE (Low Endotoxin) and EF(Endotoxin-Free) provide simple and fast method for the purification of plasmid DNA with low endotoxin contaminants.Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli.
It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. These kits use two methods for the removal of endotoxins: advanced phase separation and endotoxin removal washing.
Endotoxin levels can be reduced to 0.1 EU/㎍ with ExfectionTM EF.  Prepared plasmid DNA can be used for the transfection of most of cell lines in addition to most of molecular biological applications.
ab 302,00 EUR
zzgl. 19% MwSt. zzgl. Versand
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